Xingqun Ma¹, Shouju Wang², Yingxia Chen¹

¹PLA cancer center,Nanjing bayi hospital, ²Department of Radiology,Jinling Hospital

Objective:Photothermal therapy (PTT) has emerge as apromising way to treat cancer with minimal invasion and high selectivity. Thepurpose of this study was to synthesis gold nanoprisms (GNPs) as anew kind of photothermal agents, and test their photothermal therapeuticefficacy on breast cancers. . Method: The GNPs weresynthesised by a seed-less method and functionalized with Methoxy-polyethyleneglycol-thiol (mPEG-SH, M.W.≈5000Da).The production were characterised by transmissionelectron microscopy, UV-Vis spectrometer, and dynamic light scatteringanalyser. Breast cancer cells (MCF-7) were incubated with variousconcentration of PEGylated GNPs (from 2.5 µg to 80 µg/mL) and irradiated by a670 nm laser (2 W/cm2) for 10 min. The photothermal therapeutic efficacywas assessed by MTT assays. Result: The PEGylated GNPs with edge lengthof 55.6 ± 1.6nm was synthesised with high yield. The UV-Vis spectra of PEGylatedGNPs peaks at ~669 nm. The photothermal conversion efficacy was determined tobe 73%. MTT results showed that as the concentration of PEGylated GNPsincreased from 2.5 to 80 µg/ml, the viability of the MCF-7 cells decreased from92.2±6.3% to 5.5±1.4% of the blank control after laser irradiation. Conclusion:In conclusion, this study demonstrated that small-sized PEGylated GNPs canbe used for PTT to treat breast cancer with extremely high photothermalconversion efficacy.

Key Words: photothermal therapy  gold nanoparticles  breast cancer